Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.

@article{citeulike:1984537, abstract = {Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. Here we show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem cells. These human induced pluripotent stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such human induced pluripotent cell lines should be useful in the production of new disease models and in drug development as well as application in transplantation medicine once technical limitations (for example, mutation through viral integration) are eliminated.}, address = {Genome Center of Wisconsin, Madison, WI 53706-1580, USA.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715-1299, USA.}, author = {Yu, Junying and Vodyanik, Maxim A A. and Smuga-Otto, Kim and Antosiewicz-Bourget, Jessica and Frane, Jennifer L L. and Tian, Shulan and Nie, Jeff and Jonsdottir, Gudrun A A. and Ruotti, Victor and Stewart, Ron and Slukvin, Igor I I. and Thomson, James A A. }, citeulike-article-id = {1984537}, doi = {http://dx.doi.org/10.1126/science.1151526}, issn = {1095-9203}, journal = {Science}, month = {November}, posted-at = {2007-12-27 15:09:24}, priority = {2}, title = {Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.}, url = {http://dx.doi.org/10.1126/science.1151526}, year = {2007} }

TY - JOUR ID - citeulike:1984537 L3 - citeulike-article-id:1984537 TI - Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. JF - Science AD - Genome Center of Wisconsin, Madison, WI 53706-1580, USA.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715-1299, USA. SN - 1095-9203 N2 - Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. Here we show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem cells. These human induced pluripotent stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such human induced pluripotent cell lines should be useful in the production of new disease models and in drug development as well as application in transplantation medicine once technical limitations (for example, mutation through viral integration) are eliminated. AU - Yu, Junying AU - Vodyanik, Maxim A AU - Smuga-Otto, Kim AU - Antosiewicz-Bourget, Jessica AU - Frane, Jennifer L AU - Tian, Shulan AU - Nie, Jeff AU - Jonsdottir, Gudrun A AU - Ruotti, Victor AU - Stewart, Ron AU - Slukvin, Igor I AU - Thomson, James A PY - 2007/11/20/ UR - http://dx.doi.org/10.1126/science.1151526 ER -