Cell water dynamics on multiple time scales

@article{citeulike:2735024, abstract = {Water-biomolecule interactions have been extensively studied in dilute solutions, crystals, and rehydrated powders, but none of these model systems may capture the behavior of water in the highly organized intracellular milieu. Because of the experimental difficulty of selectively probing the structure and dynamics of water in intact cells, radically different views about the properties of cell water have proliferated. To resolve this long-standing controversy, we have measured the 2H spin relaxation rate in living bacteria cultured in D2O. The relaxation data, acquired in a wide magnetic field range (0.2 mT-12 T) and analyzed in a model-independent way, reveal water dynamics on a wide range of time scales. Contradicting the view that a substantial fraction of cell water is strongly perturbed, we find that {approx}85\% of cell water in Escherichia coli and in the extreme halophile Haloarcula marismortui has bulk-like dynamics. The remaining {approx}15\% of cell water interacts directly with biomolecular surfaces and is motionally retarded by a factor 15 {+/-} 3 on average, corresponding to a rotational correlation time of 27 ps. This dynamic perturbation is three times larger than for small monomeric proteins in solution, a difference we attribute to secluded surface hydration sites in supramolecular assemblies. The relaxation data also show that a small fraction ({approx}0.1\%) of cell water exchanges from buried hydration sites on the microsecond time scale, consistent with the current understanding of protein hydration in solutions and crystals. 10.1073/pnas.0709585105}, author = {Persson, Erik and Halle, Bertil }, citeulike-article-id = {2735024}, doi = {10.1073/pnas.0709585105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {dynamics, toread}, month = {April}, number = {17}, pages = {6266--6271}, posted-at = {2008-04-29 18:40:51}, priority = {2}, title = {Cell water dynamics on multiple time scales}, url = {http://dx.doi.org/10.1073/pnas.0709585105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:2735024 L3 - citeulike-article-id:2735024 TI - Cell water dynamics on multiple time scales JF - Proceedings of the National Academy of Sciences VL - 105 IS - 17 SP - 6266 EP - 6271 N2 - Water-biomolecule interactions have been extensively studied in dilute solutions, crystals, and rehydrated powders, but none of these model systems may capture the behavior of water in the highly organized intracellular milieu. Because of the experimental difficulty of selectively probing the structure and dynamics of water in intact cells, radically different views about the properties of cell water have proliferated. To resolve this long-standing controversy, we have measured the 2H spin relaxation rate in living bacteria cultured in D2O. The relaxation data, acquired in a wide magnetic field range (0.2 mT-12 T) and analyzed in a model-independent way, reveal water dynamics on a wide range of time scales. Contradicting the view that a substantial fraction of cell water is strongly perturbed, we find that {approx}85% of cell water in Escherichia coli and in the extreme halophile Haloarcula marismortui has bulk-like dynamics. The remaining {approx}15% of cell water interacts directly with biomolecular surfaces and is motionally retarded by a factor 15 {+/-} 3 on average, corresponding to a rotational correlation time of 27 ps. This dynamic perturbation is three times larger than for small monomeric proteins in solution, a difference we attribute to secluded surface hydration sites in supramolecular assemblies. The relaxation data also show that a small fraction ({approx}0.1%) of cell water exchanges from buried hydration sites on the microsecond time scale, consistent with the current understanding of protein hydration in solutions and crystals. 10.1073/pnas.0709585105 KW - dynamics KW - toread AU - Persson, Erik AU - Halle, Bertil PY - 2008/04/29/ UR - http://dx.doi.org/10.1073/pnas.0709585105 ER -