Chromosomal distribution and coding capacity of the human endogenous retrovirus HERV-W family.

@article{citeulike:2808868, abstract = {Some genomic elements of the multicopy HERV-W endogenous retroviral family have been previously identified in databases. One of them, located on chromosome 7, contains a single complete open reading frame (ORF) putatively encoding an envelope protein. We have experimentally investigated the genomic complexity and coding capacity of the HERV-W family. The human haploid genome contains at least 70, 100, and 30 HERV-W-related gag, pro, and env regions, respectively, widely and heterogeneously dispersed among chromosomes. Using in vitro transcription-translation procedures, three putative HERV-W gag, pro, and env ORFs were detected on chromosomes 3, 6, and 7, respectively, and their sequences analyzed. A 363 amino acid gag ORF containing matrix and carboxy-terminal truncated capsid domains encoded a putative 45-kDa protein. No gag-pro ORF was found, but a pro sequence containing a DTG active site was detected. Finally, the previously described 538 amino acid HERV-W env ORF, located on chromosome 7, was shown to be unique and encoded a putative 80-kDa glycosylated protein. Proteins of molecular mass identical to the one obtained by an in vitro transcription-translation procedure were detected in human placenta, using anti HERV-W Gag- and Env-specific antibodies. The absence of an HERV-W replication-competent provirus versus the existence of HERV-W-related Gag and Env proteins in healthy human placenta is discussed with respect to particle formation, physiology, and pathology.}, address = {Unit\'{e} Mixte 103 CNRS-bioM\'{e}rieux, Ecole Normale Sup\'{e}rieure de Lyon, France.}, author = {Voisset, C. and Bouton, O. and Bedin, F. and Duret, L. and Mandrand, B. and Mallet, F. and Paranhos-Baccala, G. }, citeulike-article-id = {2808868}, doi = {10.1089/088922200308738}, issn = {0889-2229}, journal = {AIDS research and human retroviruses}, month = {May}, number = {8}, pages = {731--740}, posted-at = {2008-07-01 11:53:27}, priority = {0}, title = {Chromosomal distribution and coding capacity of the human endogenous retrovirus HERV-W family.}, url = {http://dx.doi.org/10.1089/088922200308738}, volume = {16}, year = {2000} }

TY - JOUR ID - citeulike:2808868 L3 - citeulike-article-id:2808868 TI - Chromosomal distribution and coding capacity of the human endogenous retrovirus HERV-W family. JF - AIDS research and human retroviruses VL - 16 IS - 8 SP - 731 EP - 740 AD - Unité Mixte 103 CNRS-bioMérieux, Ecole Normale Supérieure de Lyon, France. SN - 0889-2229 N2 - Some genomic elements of the multicopy HERV-W endogenous retroviral family have been previously identified in databases. One of them, located on chromosome 7, contains a single complete open reading frame (ORF) putatively encoding an envelope protein. We have experimentally investigated the genomic complexity and coding capacity of the HERV-W family. The human haploid genome contains at least 70, 100, and 30 HERV-W-related gag, pro, and env regions, respectively, widely and heterogeneously dispersed among chromosomes. Using in vitro transcription-translation procedures, three putative HERV-W gag, pro, and env ORFs were detected on chromosomes 3, 6, and 7, respectively, and their sequences analyzed. A 363 amino acid gag ORF containing matrix and carboxy-terminal truncated capsid domains encoded a putative 45-kDa protein. No gag-pro ORF was found, but a pro sequence containing a DTG active site was detected. Finally, the previously described 538 amino acid HERV-W env ORF, located on chromosome 7, was shown to be unique and encoded a putative 80-kDa glycosylated protein. Proteins of molecular mass identical to the one obtained by an in vitro transcription-translation procedure were detected in human placenta, using anti HERV-W Gag- and Env-specific antibodies. The absence of an HERV-W replication-competent provirus versus the existence of HERV-W-related Gag and Env proteins in healthy human placenta is discussed with respect to particle formation, physiology, and pathology. AU - Voisset, C AU - Bouton, O AU - Bedin, F AU - Duret, L AU - Mandrand, B AU - Mallet, F AU - Paranhos-Baccala, G PY - 2000/05/20/ UR - http://dx.doi.org/10.1089/088922200308738 ER -