Ookinete antigens of Plasmodium berghei. Appearance on the zygote surface of an Mr 21 kD determinant identified by transmission-blocking monoclonal antibodies.

@article{citeulike:1123081, abstract = {Zygotes and ookinetes of the rodent malaria Plasmodium berghei can be enriched 50-fold, from whole blood cultures by ammonium chloride lysis. Three monoclonal antibodies (MoAbs) raised against such enriched preparations specifically bind to a determinant of Mr 21 kD as assessed by 125I-labelled goat anti-mouse IgG probed immunoblots of Western transfers of SDS-PAGE gels. Indirect immunofluorescence indicates that the 21 kD determinant bound by specific MoAbs, whilst not detectable on gametocytes or gametes, appears on the parasite surface within 2 h of exflagellation/fertilization and increases thereafter. The three MoAbs specifically binding the 21 kD determinant block oocyst development in mosquitoes by at least 90\%, as assessed either by in-vitro membrane feeds or by live feeds on passively immunized mice. These MoAbs reduce ookinete formation in vitro by between 52 and 100\%. Possible mechanisms of action of these MoAbs are discussed.}, address = {Department of Pure and Applied Biology, Imperial College of Science and Technology, London.}, author = {Winger, L. A. and Tirawanchai, N. and Nicholas, J. and Carter, H. E. and Smith, J. E. and Sinden, R. E. }, citeulike-article-id = {1123081}, issn = {0141-9838}, journal = {Parasite Immunol}, keywords = {p21}, month = {March}, number = {2}, pages = {193--207}, posted-at = {2007-02-26 15:07:26}, priority = {2}, title = {Ookinete antigens of Plasmodium berghei. Appearance on the zygote surface of an Mr 21 kD determinant identified by transmission-blocking monoclonal antibodies.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2453831}, volume = {10}, year = {1988} }

TY - JOUR ID - citeulike:1123081 L3 - citeulike-article-id:1123081 TI - Ookinete antigens of Plasmodium berghei. Appearance on the zygote surface of an Mr 21 kD determinant identified by transmission-blocking monoclonal antibodies. JF - Parasite Immunol VL - 10 IS - 2 SP - 193 EP - 207 AD - Department of Pure and Applied Biology, Imperial College of Science and Technology, London. SN - 0141-9838 N2 - Zygotes and ookinetes of the rodent malaria Plasmodium berghei can be enriched 50-fold, from whole blood cultures by ammonium chloride lysis. Three monoclonal antibodies (MoAbs) raised against such enriched preparations specifically bind to a determinant of Mr 21 kD as assessed by 125I-labelled goat anti-mouse IgG probed immunoblots of Western transfers of SDS-PAGE gels. Indirect immunofluorescence indicates that the 21 kD determinant bound by specific MoAbs, whilst not detectable on gametocytes or gametes, appears on the parasite surface within 2 h of exflagellation/fertilization and increases thereafter. The three MoAbs specifically binding the 21 kD determinant block oocyst development in mosquitoes by at least 90%, as assessed either by in-vitro membrane feeds or by live feeds on passively immunized mice. These MoAbs reduce ookinete formation in vitro by between 52 and 100%. Possible mechanisms of action of these MoAbs are discussed. KW - p21 AU - Winger, LA AU - Tirawanchai, N AU - Nicholas, J AU - Carter, HE AU - Smith, JE AU - Sinden, RE PY - 1988/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/2453831 ER -