Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors.

by: A Kallioniemi, OP Kallioniemi, D Sudar, D Rutovitz, JW Gray, F Waldman, D Pinkel

Science, Vol. 258, No. 5083. (30 October 1992), pp. 818-821.

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Abstract

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

@article{citeulike:2233727, abstract = {Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.}, address = {Department of Laboratory Medicine, University of California, San Francisco 94143.}, author = {Kallioniemi, A. and Kallioniemi, O. P. and Sudar, D. and Rutovitz, D. and Gray, J. W. and Waldman, F. and Pinkel, D. }, citeulike-article-id = {2233727}, issn = {0036-8075}, journal = {Science}, month = {October}, number = {5083}, pages = {818--821}, posted-at = {2008-01-15 06:02:23}, priority = {2}, title = {Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1359641}, volume = {258}, year = {1992} }

RIS record

TY - JOUR ID - citeulike:2233727 L3 - citeulike-article-id:2233727 TI - Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. JF - Science VL - 258 IS - 5083 SP - 818 EP - 821 AD - Department of Laboratory Medicine, University of California, San Francisco 94143. SN - 0036-8075 N2 - Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified. AU - Kallioniemi, A AU - Kallioniemi, OP AU - Sudar, D AU - Rutovitz, D AU - Gray, JW AU - Waldman, F AU - Pinkel, D PY - 1992/10/30/ UR - http://view.ncbi.nlm.nih.gov/pubmed/1359641 ER -

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