Patterns of Known and Novel Small RNAs in Human Cervical Cancer.

@article{citeulike:1447000, abstract = {Recent studies suggest that knowledge of differential expression of microRNAs (miRNA) in cancer may have substantial diagnostic and prognostic value. Here, we use a direct sequencing method to characterize the profiles of miRNAs and other small RNA segments for six human cervical carcinoma cell lines and five normal cervical samples. Of 166 miRNAs expressed in normal cervix and cancer cell lines, we observed significant expression variation of six miRNAs between the two groups. To further show the biological relevance of our findings, we examined the expression level of two significantly varying miRNAs in a panel of 29 matched pairs of human cervical cancer and normal cervical samples. Reduced expression of miR-143 and increased expression of miR-21 were reproducibly displayed in cancer samples, suggesting the potential value of these miRNAs as tumor markers. In addition to the known miRNAs, we found a number of novel miRNAs and an additional set of small RNAs that do not meet miRNA criteria. [Cancer Res 2007;67(13):6031-43].}, address = {Departments of Pathology, Medicine, and Genetics, Stanford University School of Medicine, Stanford, California, and Stanford Genome Technology Center, Palo Alto, California.}, author = {Lui, W. O. and Pourmand, N. and Patterson, B. K. and Fire, A. }, citeulike-article-id = {1447000}, doi = {10.1158/0008-5472.CAN-06-0561}, issn = {0008-5472}, journal = {Cancer Res}, keywords = {cancer, cervical, human, mirna}, month = {July}, number = {13}, pages = {6031--6043}, posted-at = {2007-07-10 17:20:29}, priority = {4}, title = {Patterns of Known and Novel Small RNAs in Human Cervical Cancer.}, url = {http://dx.doi.org/10.1158/0008-5472.CAN-06-0561}, volume = {67}, year = {2007} }

TY - JOUR ID - citeulike:1447000 L3 - citeulike-article-id:1447000 TI - Patterns of Known and Novel Small RNAs in Human Cervical Cancer. JF - Cancer Res VL - 67 IS - 13 SP - 6031 EP - 6043 AD - Departments of Pathology, Medicine, and Genetics, Stanford University School of Medicine, Stanford, California, and Stanford Genome Technology Center, Palo Alto, California. SN - 0008-5472 N2 - Recent studies suggest that knowledge of differential expression of microRNAs (miRNA) in cancer may have substantial diagnostic and prognostic value. Here, we use a direct sequencing method to characterize the profiles of miRNAs and other small RNA segments for six human cervical carcinoma cell lines and five normal cervical samples. Of 166 miRNAs expressed in normal cervix and cancer cell lines, we observed significant expression variation of six miRNAs between the two groups. To further show the biological relevance of our findings, we examined the expression level of two significantly varying miRNAs in a panel of 29 matched pairs of human cervical cancer and normal cervical samples. Reduced expression of miR-143 and increased expression of miR-21 were reproducibly displayed in cancer samples, suggesting the potential value of these miRNAs as tumor markers. In addition to the known miRNAs, we found a number of novel miRNAs and an additional set of small RNAs that do not meet miRNA criteria. [Cancer Res 2007;67(13):6031-43]. KW - cancer KW - cervical KW - human KW - mirna AU - Lui, WO AU - Pourmand, N AU - Patterson, BK AU - Fire, A PY - 2007/07/01/ UR - http://dx.doi.org/10.1158/0008-5472.CAN-06-0561 ER -