Intrarectal transmission, systemic infection, and CD4+ T cell depletion in humanized mice infected with HIV-1

@article{citeulike:2955395, abstract = {Intrarectal infection between men who have sex with men represents a predominant form of human immunodeficiency virus (HIV) transmission in developed countries. Currently there are no adequate small animal models that recapitulate intrarectal HIV transmission. Here we demonstrate that human lymphocytes generated in situ from hematopoietic stem cells reconstitute the gastrointestinal tract of humanized mice with human CD4+ T cells rendering them susceptible to intrarectal HIV transmission. HIV infection after a single intrarectal inoculation results in systemic infection with depletion of CD4+ T cells in gut-associated lymphoid tissue and other pathologic sequela that closely mimics those observed in HIV infected humans. This novel model provides the basis for the development and evaluation of novel approaches aimed at immune reconstitution of human gut-associated lymphoid tissue and for the development, testing, and implementation of microbicides to prevent intrarectal HIV-1 transmission. 10.1084/jem.20062411}, author = {Sun, Zhifeng and Denton, Paul W. and Estes, Jacob D. and Othieno, Florence A. and Wei, Bangdong L. and Wege, Anja K. and Melkus, Michael W. and Padgett-Thomas, Angela and Zupancic, Mary and Haase, Ashley T. and Garcia, Victor J. }, citeulike-article-id = {2955395}, doi = {http://dx.doi.org/10.1084/jem.20062411}, journal = {J. Exp. Med.}, keywords = {humanized\_mouse}, month = {March}, pages = {jem.20062411+}, posted-at = {2008-07-03 08:00:29}, priority = {2}, title = {Intrarectal transmission, systemic infection, and CD4+ T cell depletion in humanized mice infected with HIV-1}, url = {http://dx.doi.org/10.1084/jem.20062411}, year = {2007} }

TY - JOUR ID - citeulike:2955395 L3 - citeulike-article-id:2955395 TI - Intrarectal transmission, systemic infection, and CD4+ T cell depletion in humanized mice infected with HIV-1 JF - J. Exp. Med. SP - jem.20062411 N2 - Intrarectal infection between men who have sex with men represents a predominant form of human immunodeficiency virus (HIV) transmission in developed countries. Currently there are no adequate small animal models that recapitulate intrarectal HIV transmission. Here we demonstrate that human lymphocytes generated in situ from hematopoietic stem cells reconstitute the gastrointestinal tract of humanized mice with human CD4+ T cells rendering them susceptible to intrarectal HIV transmission. HIV infection after a single intrarectal inoculation results in systemic infection with depletion of CD4+ T cells in gut-associated lymphoid tissue and other pathologic sequela that closely mimics those observed in HIV infected humans. This novel model provides the basis for the development and evaluation of novel approaches aimed at immune reconstitution of human gut-associated lymphoid tissue and for the development, testing, and implementation of microbicides to prevent intrarectal HIV-1 transmission. 10.1084/jem.20062411 KW - humanized_mouse AU - Sun, Zhifeng AU - Denton, Paul AU - Estes, Jacob AU - Othieno, Florence AU - Wei, Bangdong AU - Wege, Anja AU - Melkus, Michael AU - Padgett-Thomas, Angela AU - Zupancic, Mary AU - Haase, Ashley AU - Garcia, Victor PY - 2007/03/26/ UR - http://dx.doi.org/10.1084/jem.20062411 ER -