A high-resolution map of nucleosome positioning on a fission yeast centromere

@article{citeulike:2952442, abstract = {A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe. Previous studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosomes and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are, in fact, positioned in regular intervals in the core of centromere 2, providing the first high-resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore protein genes cnp1, mis18, mis12, nuf2, mal2; overexpression of cnp1; or the deletion of ams2, which encodes a GATA-like factor participating in CENPA incorporation. Bioinformatics analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence bias in nucleosome positioning. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites of Ams2p. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence. 10.1101/gr.075374.107}, author = {Song, Jun S. and Liu, Xingkun and Liu, Shirley X. and He, Xiangwei }, citeulike-article-id = {2952442}, doi = {10.1101/gr.075374.107}, journal = {Genome Res.}, keywords = {nucleosome}, month = {July}, number = {7}, pages = {1064--1072}, posted-at = {2008-07-02 17:02:09}, priority = {2}, title = {A high-resolution map of nucleosome positioning on a fission yeast centromere}, url = {http://dx.doi.org/10.1101/gr.075374.107}, volume = {18}, year = {2008} }

TY - JOUR ID - citeulike:2952442 L3 - citeulike-article-id:2952442 TI - A high-resolution map of nucleosome positioning on a fission yeast centromere JF - Genome Res. VL - 18 IS - 7 SP - 1064 EP - 1072 N2 - A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe. Previous studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosomes and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are, in fact, positioned in regular intervals in the core of centromere 2, providing the first high-resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore protein genes cnp1, mis18, mis12, nuf2, mal2; overexpression of cnp1; or the deletion of ams2, which encodes a GATA-like factor participating in CENPA incorporation. Bioinformatics analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence bias in nucleosome positioning. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites of Ams2p. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence. 10.1101/gr.075374.107 KW - nucleosome AU - Song, Jun AU - Liu, Xingkun AU - Liu, Shirley AU - He, Xiangwei PY - 2008/07/01/ UR - http://dx.doi.org/10.1101/gr.075374.107 ER -