Analysis of yeast protein kinases using protein chips.

@article{citeulike:673488, abstract = {We have developed a novel protein chip technology that allows the high-throughput analysis of biochemical activities, and used this approach to analyse nearly all of the protein kinases from Saccharomyces cerevisiae. Protein chips are disposable arrays of microwells in silicone elastomer sheets placed on top of microscope slides. The high density and small size of the wells allows for high-throughput batch processing and simultaneous analysis of many individual samples. Only small amounts of protein are required. Of 122 known and predicted yeast protein kinases, 119 were overexpressed and analysed using 17 different substrates and protein chips. We found many novel activities and that a large number of protein kinases are capable of phosphorylating tyrosine. The tyrosine phosphorylating enzymes often share common amino acid residues that lie near the catalytic region. Thus, our study identified a number of novel features of protein kinases and demonstrates that protein chip technology is useful for high-throughput screening of protein biochemical activity.}, address = {Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, USA.}, author = {Zhu, H. and Klemic, J. F. and Chang, S. and Bertone, P. and Casamayor, A. and Klemic, K. G. and Smith, D. and Gerstein, M. and Reed, M. A. and Snyder, M. }, citeulike-article-id = {673488}, doi = {http://dx.doi.org/10.1038/81576}, issn = {1061-4036}, journal = {Nat Genet}, keywords = {chip, kinase, method}, month = {November}, number = {3}, pages = {283--289}, posted-at = {2006-05-28 17:04:29}, priority = {0}, title = {Analysis of yeast protein kinases using protein chips.}, url = {http://dx.doi.org/10.1038/81576}, volume = {26}, year = {2000} }

TY - JOUR ID - citeulike:673488 L3 - citeulike-article-id:673488 TI - Analysis of yeast protein kinases using protein chips. JF - Nat Genet VL - 26 IS - 3 SP - 283 EP - 289 AD - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, USA. SN - 1061-4036 N2 - We have developed a novel protein chip technology that allows the high-throughput analysis of biochemical activities, and used this approach to analyse nearly all of the protein kinases from Saccharomyces cerevisiae. Protein chips are disposable arrays of microwells in silicone elastomer sheets placed on top of microscope slides. The high density and small size of the wells allows for high-throughput batch processing and simultaneous analysis of many individual samples. Only small amounts of protein are required. Of 122 known and predicted yeast protein kinases, 119 were overexpressed and analysed using 17 different substrates and protein chips. We found many novel activities and that a large number of protein kinases are capable of phosphorylating tyrosine. The tyrosine phosphorylating enzymes often share common amino acid residues that lie near the catalytic region. Thus, our study identified a number of novel features of protein kinases and demonstrates that protein chip technology is useful for high-throughput screening of protein biochemical activity. KW - chip KW - kinase KW - method AU - Zhu, H AU - Klemic, JF AU - Chang, S AU - Bertone, P AU - Casamayor, A AU - Klemic, KG AU - Smith, D AU - Gerstein, M AU - Reed, MA AU - Snyder, M PY - 2000/11// UR - http://dx.doi.org/10.1038/81576 ER -