Kinesin Walks Hand-Over-Hand

by: Ahmet Yildiz, Michio Tomishige, Ronald D Vale, Paul R Selvin

Science, Vol. 303, No. 5658. (30 January 2004), pp. 676-678.

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Abstract

Kinesin is a processive motor that takes 8.3-nm center-of-mass steps along microtubules for each adenosine triphosphate hydrolyzed. Whether kinesin moves by a "hand-over-hand" or an "inchworm" model has been controversial. We have labeled a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to within 2 nm before and after a step. We observed that single kinesin heads take steps of 17.3 +/- 3.3 nm. A kinetic analysis of the dwell times between steps shows that the 17-nm steps alternate with 0-nm steps. These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps. 10.1126/science.1093753

@article{citeulike:623637, abstract = {Kinesin is a processive motor that takes 8.3-nm center-of-mass steps along microtubules for each adenosine triphosphate hydrolyzed. Whether kinesin moves by a "hand-over-hand" or an "inchworm" model has been controversial. We have labeled a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to within 2 nm before and after a step. We observed that single kinesin heads take steps of 17.3 {+/-} 3.3 nm. A kinetic analysis of the dwell times between steps shows that the 17-nm steps alternate with 0-nm steps. These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps. 10.1126/science.1093753}, author = {Yildiz, Ahmet and Tomishige, Michio and Vale, Ronald D. and Selvin, Paul R. }, citeulike-article-id = {623637}, doi = {10.1126/science.1093753}, journal = {Science}, keywords = {kinesin, motor}, month = {January}, number = {5658}, pages = {676--678}, posted-at = {2007-06-02 02:01:46}, priority = {4}, title = {Kinesin Walks Hand-Over-Hand}, url = {http://dx.doi.org/10.1126/science.1093753}, volume = {303}, year = {2004} }

RIS record

TY - JOUR ID - citeulike:623637 L3 - citeulike-article-id:623637 TI - Kinesin Walks Hand-Over-Hand JF - Science VL - 303 IS - 5658 SP - 676 EP - 678 N2 - Kinesin is a processive motor that takes 8.3-nm center-of-mass steps along microtubules for each adenosine triphosphate hydrolyzed. Whether kinesin moves by a "hand-over-hand" or an "inchworm" model has been controversial. We have labeled a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to within 2 nm before and after a step. We observed that single kinesin heads take steps of 17.3 {+/-} 3.3 nm. A kinetic analysis of the dwell times between steps shows that the 17-nm steps alternate with 0-nm steps. These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps. 10.1126/science.1093753 KW - kinesin KW - motor AU - Yildiz, Ahmet AU - Tomishige, Michio AU - Vale, Ronald AU - Selvin, Paul PY - 2004/01/30/ UR - http://dx.doi.org/10.1126/science.1093753 ER -

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