Direct observation of kinesin stepping by optical trapping interferometry

by: Karel Svoboda, Christoph F Schmidt, Bruce J Schnapp, Steven M Block

Nature, Vol. 365, No. 6448. (21 October 1993), pp. 721-727.

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Abstract

Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

@article{Svoboda1993, abstract = {Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.}, author = {Svoboda, Karel and Schmidt, Christoph F. and Schnapp, Bruce J. and Block, Steven M. }, citeulike-article-id = {1357250}, doi = {10.1038/365721a0}, journal = {Nature}, keywords = {kinesin, motor}, month = {October}, number = {6448}, pages = {721--727}, posted-at = {2007-06-02 14:48:34}, priority = {2}, title = {Direct observation of kinesin stepping by optical trapping interferometry}, url = {http://dx.doi.org/10.1038/365721a0}, volume = {365}, year = {1993} }

RIS record

TY - JOUR ID - Svoboda1993 L3 - citeulike-article-id:1357250 TI - Direct observation of kinesin stepping by optical trapping interferometry JF - Nature VL - 365 IS - 6448 SP - 721 EP - 727 N2 - Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps. KW - kinesin KW - motor AU - Svoboda, Karel AU - Schmidt, Christoph AU - Schnapp, Bruce AU - Block, Steven PY - 1993/10/21/ UR - http://dx.doi.org/10.1038/365721a0 ER -

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