Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age.

@article{citeulike:300796, abstract = {The bacterium Helicobacter pylori colonizes the gastric mucosa of half of the human population, resulting in chronic gastritis, ulcers, and cancer. We sequenced ten gene fragments from pairs of strains isolated sequentially at a mean interval of 1.8 years from 26 individuals. Several isolates had acquired small mosaic segments from other H. pylori or point mutations. The maximal mutation rate, the import size, and the frequency of recombination were calculated by using a Bayesian model. The calculations indicate that the last common ancestor of H. pylori existed at least 2,500-11,000 years ago. Imported mosaics have a median size of 417 bp, much smaller than for other bacteria, and recombination occurs frequently (60 imports spanning 25,000 bp per genome per year). Thus, the panmictic population structure of H. pylori results from very frequent recombination during mixed colonization by unrelated strains.}, address = {Max-Planck Institut f\"{u}r Infektionsbiologie, Schumannstrasse 21/22, 10117 Berlin, Germany.}, author = {Falush, D. and Kraft, C. and Taylor, N. S. and Correa, P. and Fox, J. G. and Achtman, M. and Suerbaum, S. }, citeulike-article-id = {300796}, doi = {10.1073/pnas.251396098}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, month = {December}, number = {26}, pages = {15056--15061}, posted-at = {2007-08-03 11:40:47}, priority = {2}, title = {Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age.}, url = {http://dx.doi.org/10.1073/pnas.251396098}, volume = {98}, year = {2001} }

TY - JOUR ID - citeulike:300796 L3 - citeulike-article-id:300796 TI - Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age. JF - Proc Natl Acad Sci U S A VL - 98 IS - 26 SP - 15056 EP - 15061 AD - Max-Planck Institut für Infektionsbiologie, Schumannstrasse 21/22, 10117 Berlin, Germany. SN - 0027-8424 N2 - The bacterium Helicobacter pylori colonizes the gastric mucosa of half of the human population, resulting in chronic gastritis, ulcers, and cancer. We sequenced ten gene fragments from pairs of strains isolated sequentially at a mean interval of 1.8 years from 26 individuals. Several isolates had acquired small mosaic segments from other H. pylori or point mutations. The maximal mutation rate, the import size, and the frequency of recombination were calculated by using a Bayesian model. The calculations indicate that the last common ancestor of H. pylori existed at least 2,500-11,000 years ago. Imported mosaics have a median size of 417 bp, much smaller than for other bacteria, and recombination occurs frequently (60 imports spanning 25,000 bp per genome per year). Thus, the panmictic population structure of H. pylori results from very frequent recombination during mixed colonization by unrelated strains. AU - Falush, D AU - Kraft, C AU - Taylor, NS AU - Correa, P AU - Fox, JG AU - Achtman, M AU - Suerbaum, S PY - 2001/12/18/ UR - http://dx.doi.org/10.1073/pnas.251396098 ER -