Food deprivation decreases mRNA and activity of the rat dopamine transporter.

@article{citeulike:2765631, abstract = {We have hypothesized that the midbrain dopamine (DA) neurons are a target for insulin action in the central nervous system (CNS). In support of this hypothesis, we have previously demonstrated that direct intracerebroventricular infusion of insulin results in an increase in mRNA levels for the DA reuptake transporter (DAT). In this study, 24- to 36-hour food deprivation was used as a model of decreased CNS insulin levels, to test whether DAT mRNA levels, DAT protein concentration or DAT functional activity would be decreased. DAT mRNA levels, assessed by in situ hybridization, were significantly decreased in the ventral tegmental area/substantia nigra pars compacta (VTA/SNc) (77 +/- 7\% of controls, p < 0.05) of food-deprived (hypoinsulinemic) rats. Binding of a specific high-affinity DAT ligand (125I-RTI-121) to membranes from brain regions of fasted or free-feeding rats provided an estimate of DAT protein, which was unchanged in both of the major terminal projection fields, the striatum and nucleus accumbens (NAc). In addition, we utilized the rotating disk electrode voltametry technique to assess possible changes in the function of the DAT in fasting (hypoinsulinemic) rats. The Vmax of DA uptake was significantly decreased (87 +/- 7\% of control, p < 0.05), without a change in the Km of uptake, in striatum from fasted rats. In vitro incubation with a physiological concentration (1 nM) of insulin resulted in an increase of striatal DA uptake to control levels. We conclude that striatal DAT function can be modulated by fasting and nutritional status, with a contribution by insulin.}, address = {Department of Psychology, University of Washington, Seattle, USA.}, author = {Patterson, T. A. and Brot, M. D. and Zavosh, A. and Schenk, J. O. and Szot, P. and Figlewicz, D. P. }, citeulike-article-id = {2765631}, issn = {0028-3835}, journal = {Neuroendocrinology}, keywords = {dopamine, eating, insulin, rat}, month = {July}, number = {1}, pages = {11--20}, posted-at = {2008-05-07 13:18:34}, priority = {2}, title = {Food deprivation decreases mRNA and activity of the rat dopamine transporter.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9695934}, volume = {68}, year = {1998} }

TY - JOUR ID - citeulike:2765631 L3 - citeulike-article-id:2765631 TI - Food deprivation decreases mRNA and activity of the rat dopamine transporter. JF - Neuroendocrinology VL - 68 IS - 1 SP - 11 EP - 20 AD - Department of Psychology, University of Washington, Seattle, USA. SN - 0028-3835 N2 - We have hypothesized that the midbrain dopamine (DA) neurons are a target for insulin action in the central nervous system (CNS). In support of this hypothesis, we have previously demonstrated that direct intracerebroventricular infusion of insulin results in an increase in mRNA levels for the DA reuptake transporter (DAT). In this study, 24- to 36-hour food deprivation was used as a model of decreased CNS insulin levels, to test whether DAT mRNA levels, DAT protein concentration or DAT functional activity would be decreased. DAT mRNA levels, assessed by in situ hybridization, were significantly decreased in the ventral tegmental area/substantia nigra pars compacta (VTA/SNc) (77 +/- 7% of controls, p < 0.05) of food-deprived (hypoinsulinemic) rats. Binding of a specific high-affinity DAT ligand (125I-RTI-121) to membranes from brain regions of fasted or free-feeding rats provided an estimate of DAT protein, which was unchanged in both of the major terminal projection fields, the striatum and nucleus accumbens (NAc). In addition, we utilized the rotating disk electrode voltametry technique to assess possible changes in the function of the DAT in fasting (hypoinsulinemic) rats. The Vmax of DA uptake was significantly decreased (87 +/- 7% of control, p < 0.05), without a change in the Km of uptake, in striatum from fasted rats. In vitro incubation with a physiological concentration (1 nM) of insulin resulted in an increase of striatal DA uptake to control levels. We conclude that striatal DAT function can be modulated by fasting and nutritional status, with a contribution by insulin. KW - dopamine KW - eating KW - insulin KW - rat AU - Patterson, TA AU - Brot, MD AU - Zavosh, A AU - Schenk, JO AU - Szot, P AU - Figlewicz, DP PY - 1998/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/9695934 ER -