Advances in preservation methods: keeping biosensor microorganisms alive and active

@article{citeulike:2362342, abstract = {The ability of bacteria to sense their surroundings can be employed to measure the bioavailability and toxicity of pollutants. However, long-term maintenance of both viability and activity of the sensor bacteria is required for the development of cell-based devices for environmental monitoring. To meet these demands, various techniques to conserve such bacteria have been reported, including freeze drying, vacuum drying, continuous cultivation, and immobilisation in biocompatible polymers of organic or inorganic origin. Much effort has been invested in merging these bacterial preservation schemes with the construction of sensor cell arrays on platforms such as biochips or optic fibres, hopefully leading to effective miniaturised whole-cell biosensor systems. These approaches hold much promise for the future. Nevertheless, their eventual implementation in practical devices calls for significant enhancement of current knowledge on formulation of reporter microorganisms.}, author = {Bjerketorp, Joakim and Hakansson, Sebastian and Belkin, Shimshon and Jansson, Janet K. }, booktitle = {Analytical biotechnology - Edited by Jan Roelof van der Meer and J Colin Murrel}, citeulike-article-id = {2362342}, doi = {10.1016/j.copbio.2005.12.005}, journal = {Current Opinion in Biotechnology}, keywords = {biosensor}, month = {February}, number = {1}, pages = {43--49}, posted-at = {2008-02-11 11:29:33}, priority = {2}, title = {Advances in preservation methods: keeping biosensor microorganisms alive and active}, url = {http://dx.doi.org/10.1016/j.copbio.2005.12.005}, volume = {17}, year = {2006} }

TY - JOUR ID - citeulike:2362342 L3 - citeulike-article-id:2362342 TI - Advances in preservation methods: keeping biosensor microorganisms alive and active T2 - Analytical biotechnology - Edited by Jan Roelof van der Meer and J Colin Murrel JF - Current Opinion in Biotechnology VL - 17 IS - 1 SP - 43 EP - 49 N2 - The ability of bacteria to sense their surroundings can be employed to measure the bioavailability and toxicity of pollutants. However, long-term maintenance of both viability and activity of the sensor bacteria is required for the development of cell-based devices for environmental monitoring. To meet these demands, various techniques to conserve such bacteria have been reported, including freeze drying, vacuum drying, continuous cultivation, and immobilisation in biocompatible polymers of organic or inorganic origin. Much effort has been invested in merging these bacterial preservation schemes with the construction of sensor cell arrays on platforms such as biochips or optic fibres, hopefully leading to effective miniaturised whole-cell biosensor systems. These approaches hold much promise for the future. Nevertheless, their eventual implementation in practical devices calls for significant enhancement of current knowledge on formulation of reporter microorganisms. KW - biosensor AU - Bjerketorp, Joakim AU - Hakansson, Sebastian AU - Belkin, Shimshon AU - Jansson, Janet PY - 2006/02// UR - http://dx.doi.org/10.1016/j.copbio.2005.12.005 ER -