Embryonic stem cells express neuronal properties in vitro.

@article{citeulike:2997636, abstract = {Mouse embryonic stem (ES) cells cultured as aggregates and exposed to retinoic acid are induced to express multiple phenotypes normally associated with neurons. A large percentage of treated aggregates produce a rich neuritic outgrowth. Dissociating the induced aggregates with trypsin and plating the cells as a monolayer results in cultures in which a sizable percentage of the cells have a neuronal appearance. These neuron-like cells express class III beta-tubulin and the neurofilament M subunit. Induced cultures express transcripts for neural-associated genes including the neurofilament L subunit, glutamate receptor subunits, the transcription factor Brn-3, and GFAP. Levels of neurofilament L and GAD67 and GAD65 transcripts rise dramatically upon induction. Physiological studies show that the neuron-like cells generate action potentials and express TTX-sensitive sodium channels, as well as voltage-gated potassium channels and calcium channels. We conclude that a complex system of neuronal gene expression can be activated in cultured ES cells. This system should be favorable for investigating some of the mechanisms that regulate neuronal differentiation.}, address = {Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.}, author = {Bain, G. and Kitchens, D. and Yao, M. and Huettner, J. E. and Gottlieb, D. I. }, citeulike-article-id = {2997636}, doi = {10.1006/dbio.1995.1085}, issn = {0012-1606}, journal = {Developmental biology}, keywords = {differentiation, neuron}, month = {April}, number = {2}, pages = {342--357}, posted-at = {2008-07-14 00:42:56}, priority = {2}, title = {Embryonic stem cells express neuronal properties in vitro.}, url = {http://dx.doi.org/10.1006/dbio.1995.1085}, volume = {168}, year = {1995} }

TY - JOUR ID - citeulike:2997636 L3 - citeulike-article-id:2997636 TI - Embryonic stem cells express neuronal properties in vitro. JF - Developmental biology VL - 168 IS - 2 SP - 342 EP - 357 AD - Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. SN - 0012-1606 N2 - Mouse embryonic stem (ES) cells cultured as aggregates and exposed to retinoic acid are induced to express multiple phenotypes normally associated with neurons. A large percentage of treated aggregates produce a rich neuritic outgrowth. Dissociating the induced aggregates with trypsin and plating the cells as a monolayer results in cultures in which a sizable percentage of the cells have a neuronal appearance. These neuron-like cells express class III beta-tubulin and the neurofilament M subunit. Induced cultures express transcripts for neural-associated genes including the neurofilament L subunit, glutamate receptor subunits, the transcription factor Brn-3, and GFAP. Levels of neurofilament L and GAD67 and GAD65 transcripts rise dramatically upon induction. Physiological studies show that the neuron-like cells generate action potentials and express TTX-sensitive sodium channels, as well as voltage-gated potassium channels and calcium channels. We conclude that a complex system of neuronal gene expression can be activated in cultured ES cells. This system should be favorable for investigating some of the mechanisms that regulate neuronal differentiation. KW - differentiation KW - neuron AU - Bain, G AU - Kitchens, D AU - Yao, M AU - Huettner, JE AU - Gottlieb, DI PY - 1995/04// UR - http://dx.doi.org/10.1006/dbio.1995.1085 ER -