Quorum-Sensing Regulation of the Biofilm Matrix Genes (pel) of Pseudomonas aeruginosa

by: Yumiko Sakuragi, Roberto Kolter

J. Bacteriol., Vol. 189, No. 14. (15 July 2007), pp. 5383-5386.

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Abstract

Quorum sensing (QS) has been previously shown to play an important role in the development of Pseudomonas aeruginosa biofilms (D. G. Davies et al., Science 280:295-298, 1998). Although QS regulation of swarming and DNA release has been shown to play important roles in biofilm development, regulation of genes directly involved in biosynthesis of biofilm matrix has not been described. Here, transcription of the pel operon, essential for the production of a glucose-rich matrix exopolysaccharide, is shown to be greatly reduced in lasI and rhlI mutants. Chemical complementation of the lasI mutant with 3-oxo-dodecanoyl homoserine lactone restores pel transcription to the wild-type level and biofilm formation ability. These findings thus connect QS signaling and transcription of genes responsible for biofilm matrix biosynthesis. 10.1128/JB.00137-07

@article{citeulike:1544855, abstract = {Quorum sensing (QS) has been previously shown to play an important role in the development of Pseudomonas aeruginosa biofilms (D. G. Davies et al., Science 280:295-298, 1998). Although QS regulation of swarming and DNA release has been shown to play important roles in biofilm development, regulation of genes directly involved in biosynthesis of biofilm matrix has not been described. Here, transcription of the pel operon, essential for the production of a glucose-rich matrix exopolysaccharide, is shown to be greatly reduced in lasI and rhlI mutants. Chemical complementation of the lasI mutant with 3-oxo-dodecanoyl homoserine lactone restores pel transcription to the wild-type level and biofilm formation ability. These findings thus connect QS signaling and transcription of genes responsible for biofilm matrix biosynthesis. 10.1128/JB.00137-07}, author = {Sakuragi, Yumiko and Kolter, Roberto }, citeulike-article-id = {1544855}, doi = {http://dx.doi.org/10.1128/JB.00137-07}, journal = {J. Bacteriol.}, keywords = {biofilm, pseudomonas, quorum-sensing}, month = {July}, number = {14}, pages = {5383--5386}, posted-at = {2007-08-09 03:36:43}, priority = {2}, title = {Quorum-Sensing Regulation of the Biofilm Matrix Genes (pel) of Pseudomonas aeruginosa}, url = {http://dx.doi.org/10.1128/JB.00137-07}, volume = {189}, year = {2007} }

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TY - JOUR ID - citeulike:1544855 L3 - citeulike-article-id:1544855 TI - Quorum-Sensing Regulation of the Biofilm Matrix Genes (pel) of Pseudomonas aeruginosa JF - J. Bacteriol. VL - 189 IS - 14 SP - 5383 EP - 5386 N2 - Quorum sensing (QS) has been previously shown to play an important role in the development of Pseudomonas aeruginosa biofilms (D. G. Davies et al., Science 280:295-298, 1998). Although QS regulation of swarming and DNA release has been shown to play important roles in biofilm development, regulation of genes directly involved in biosynthesis of biofilm matrix has not been described. Here, transcription of the pel operon, essential for the production of a glucose-rich matrix exopolysaccharide, is shown to be greatly reduced in lasI and rhlI mutants. Chemical complementation of the lasI mutant with 3-oxo-dodecanoyl homoserine lactone restores pel transcription to the wild-type level and biofilm formation ability. These findings thus connect QS signaling and transcription of genes responsible for biofilm matrix biosynthesis. 10.1128/JB.00137-07 KW - biofilm KW - pseudomonas KW - quorum-sensing AU - Sakuragi, Yumiko AU - Kolter, Roberto PY - 2007/07/15/ UR - http://dx.doi.org/10.1128/JB.00137-07 ER -

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