Thu, 21 Aug 2008 15:32:28 BST CiteULike: msuarezdiez Berg http://www.citeulike.org/user/msuarezdiez/author/Berg CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/msuarezdiez/article/933568 <i>Proc Natl Acad Sci U S A, Vol. 89, No. 16. (15 August 1992), pp. 7345-7349.</i><br /><br />Zinc finger proteins of the Cys2-His2 type consist of tandem arrays of domains, where each domain appears to contact three adjacent base pairs of DNA through three key residues. We have designed and prepared a series of variants of the central zinc finger within the DNA binding domain of Sp1 by using information from an analysis of a large data base of zinc finger protein sequences. Through systematic variations at two of the three contact positions (underlined), relatively specific recognition of sequences of the form 5'-GGGGN(G or T)GGG-3' has been achieved. These results provide the basis for rules that may develop into a code that will allow the design of zinc finger proteins with preselected DNA site specificity. Toward rules relating zinc finger protein sequences and DNA binding site preferences. JR Desjarlais JM Berg Proc Natl Acad Sci U S A, Vol. 89, No. 16. (15 August 1992), pp. 7345-7349. 2006-11-06T15:34:38-00:00 1992 Proc Natl Acad Sci U S A 0027-8424 89 16 7345 7349 zinc_fingers