Thu, 21 Aug 2008 15:36:12 BST CiteULike: lechristophe Backmann http://www.citeulike.org/user/lechristophe/author/Backmann CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/lechristophe/article/910621 <i>Nature Methods, Vol. 3, No. 11. (23 October 2006), pp. 887-889.</i><br /><br />We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion. Targeting and tracing antigens in live cells with fluorescent nanobodies Ulrich Rothbauer Kourosh Zolghadr Sergei Tillib Danny Nowak Lothar Schermelleh Anja Gahl Natalija Backmann Katja Conrath Serge Muyldermans Cristina Cardoso Heinrich Leonhardt doi:10.1038/nmeth953 Nature Methods, Vol. 3, No. 11. (23 October 2006), pp. 887-889. 2006-10-24T00:18:48-00:00 2006 Nature Methods 1548-7091 3 11 887 889 Nature Publishing Group intracellular live_cell_imaging protein_labeling technique