Thu, 24 Jul 2008 19:45:16 BST CiteULike: jyuh Kayser http://www.citeulike.org/user/jyuh/author/Kayser CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/jyuh/article/2997796 <i>International journal of legal medicine (2 July 2008)</i><br /><br />In forensic science, the unequivocal identification of the cellular origin of crime scene samples used for DNA profiling can provide crucial information for crime scene reconstruction. We have previously shown that various mRNA markers from genes with expression patterns specific for blood and saliva can be established from whole-genome expression analysis of time-wise degraded samples and were stable enough to specifically identify blood and saliva stains up to 180 days of age. Here, we showed that nine blood-specific and five saliva-specific mRNA markers can be amplified successfully and reliably in much older blood (13-16 years) and saliva (2-6 years) stains, respectively, suggesting their suitability for tissue identification in forensic case work. Moreover, our findings imply that forensic RNA testing can be reliable and robust if degraded samples are considered in the marker ascertainment procedure, with promising expectations beyond tissue identification purposes. New markers for old stains: stable mRNA markers for blood and saliva identification from up to 16-year-old stains. Dmitry Zubakov Mieke Kokshoorn Ate Kloosterman Manfred Kayser doi:10.1007/s00414-008-0249-z International journal of legal medicine (2 July 2008) 2008-07-14T02:42:35-00:00 2008 International journal of legal medicine 0937-9827 blood ffpe microarray saliva http://www.citeulike.org/user/jyuh/article/2782230 <i>Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, Vol. 28, No. 1. (February 2006), pp. 27-38.</i><br /><br />OBJECTIVE: To develop and implement an Internet-based, automated image measurement system for immunohistochemically stained slides including fluorescence images in online and off-line modes. STUDY DESIGN: An image analyzing system was developed that automatically measures digitized images obtained from immunohistochemically stained slides. It is divided into a common server platform and a specific image quantification system based upon DIAS (University of Jena). After registration, the user fills in an input data form and attaches images to be measured. The server periodically transfers the data to the measurement system. The measurement works on dynamic thresholding and active sampling of objects visualized by fluorescence and conventional chromogens. It includes stereologic algorithms, object quantification, syntactic structure analysis and quality assurance. RESULTS: The system has been tested for diaminobenzidene, alkaline phosphatase and fluorescence images (FITC, etc.). The reproducibility and stability of the system are > 98%. The series of successfully measured images comprises > 1,000 images in total in the online and off-line modes. CONCLUSION: An Internet-based automated image measurement system has been developed that offers worldwide access to the major requests for quantification of immunohistochemically stained slides-tissue array analysis, nuclear stains (MIB, hormones), membrane stains (CerbB2), vascularization and fluorescence in situ hybridization. Theory and implementation of an electronic, automated measurement system for images obtained from immunohistochemically stained slides. G Kayser D Radziszowski P Bzdyl R Sommer K Kayser Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, Vol. 28, No. 1. (February 2006), pp. 27-38. 2008-05-10T01:29:46-00:00 2006 Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology 0884-6812 28 1 27 38 no-tag http://www.citeulike.org/user/jyuh/article/2688892 <i>Diagnostic Pathology, Vol. 3 (18 April 2008), 17.</i> Image standards in Tissue-Based Diagnosis (Diagnostic Surgical Pathology) Klaus Kayser Jurgen Gortler Torsten Goldmann Ekkehard Vollmer Peter Hufnagl Gian Kayser doi:10.1186/1746-1596-3-17 Diagnostic Pathology, Vol. 3 (18 April 2008), 17. 2008-04-18T18:06:55-00:00 2008 Diagnostic Pathology 1746-1596 3 17 no-tag http://www.citeulike.org/user/jyuh/article/1815515 <i>Anal Biochem, Vol. 353, No. 2. (15 June 2006), pp. 272-277.</i><br /><br />A simple noninvasive procedure for saliva sample collection and DNA extraction was developed. On average, the amount of human DNA (as measured by a TaqMan-based assay) was about 11.4 microg/mL saliva, which is more than can be obtained from other noninvasive samples such as cheek swabs. However, the presence of large amounts of nonhuman DNA (up to 90% of the total extracted DNA) in saliva samples does necessitate DNA quantitation methods that are specific for human DNA. We were able to reliably and accurately type different genetic markers (mDNA sequences, Y-chromosomal single-nucleotide polymorphisms, and autosomal microsatellite loci) from saliva samples stored for up to 30 days at 37 degrees C, making this method well-suited for field conditions and convenient transportation of samples back to the laboratory. Thus, saliva can be considered a reliable source of DNA for a wide variety of genetic studies. Evaluation of saliva as a source of human DNA for population and association studies. D Quinque R Kittler M Kayser M Stoneking I Nasidze doi:10.1016/j.ab.2006.03.021 Anal Biochem, Vol. 353, No. 2. (15 June 2006), pp. 272-277. 2007-10-24T14:09:52-00:00 2006 Anal Biochem 0003-2697 353 2 272 277 no-tag