Thu, 21 Aug 2008 15:21:06 BST CiteULike: jyuh Drisdel http://www.citeulike.org/user/jyuh/author/Drisdel CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/jyuh/article/2332867 <i>Methods, Vol. 40, No. 2. (October 2006), pp. 127-134.</i><br /><br />Protein palmitoylation plays an important role in the structure and function of a wide array of proteins. Unlike other lipid modifications, protein palmitoylation is highly dynamic and cycles of palmitoylation and depalmitoylation can regulate protein function and localization. The dynamic nature of palmitoylation is poorly resolved because of limitations in assay methods. Here, we discuss various methods that can be used to measure protein palmitoylation and identify sites of palmitoylation. We describe new methodology based on "fatty acyl exchange labeling" in which palmitate is removed via hydroxylamine-mediated cleavage of the palmitoyl-thioester bond and then exchanged with a sulfhydryl-specific labeling compound. The techniques are highly sensitive and allow for quantitative estimates of palmitoylation. Unlike other techniques used to assay posttranslational modifications, the techniques we have developed can label all sites of modification with a variety of probes, radiolabeled or non-radioactive, and can be used to assay the palmitoylation of proteins from tissue samples. Assays of protein palmitoylation. RC Drisdel JK Alexander A Sayeed WN Green doi:10.1016/j.ymeth.2006.04.015 Methods, Vol. 40, No. 2. (October 2006), pp. 127-134. 2008-02-05T01:40:39-00:00 2006 Methods 1046-2023 40 2 127 134 no-tag http://www.citeulike.org/user/jyuh/article/1457376 <i>Methods, Vol. 40, No. 2. (October 2006), pp. 127-134.</i><br /><br />Protein palmitoylation plays an important role in the structure and function of a wide array of proteins. Unlike other lipid modifications, protein palmitoylation is highly dynamic and cycles of palmitoylation and depalmitoylation can regulate protein function and localization. The dynamic nature of palmitoylation is poorly resolved because of limitations in assay methods. Here, we discuss various methods that can be used to measure protein palmitoylation and identify sites of palmitoylation. We describe new methodology based on "fatty acyl exchange labeling" in which palmitate is removed via hydroxylamine-mediated cleavage of the palmitoyl-thioester bond and then exchanged with a sulfhydryl-specific labeling compound. The techniques are highly sensitive and allow for quantitative estimates of palmitoylation. Unlike other techniques used to assay posttranslational modifications, the techniques we have developed can label all sites of modification with a variety of probes, radiolabeled or non-radioactive, and can be used to assay the palmitoylation of proteins from tissue samples. Assays of protein palmitoylation Renaldo Drisdel John Alexander Ayaz Sayeed William Green Methods, Vol. 40, No. 2. (October 2006), pp. 127-134. 2007-07-15T09:18:26-00:00 2006 Methods 40 2 127 134 no-tag