Thu, 24 Jul 2008 19:49:08 BST CiteULike: brembs aplysia http://www.citeulike.org/user/brembs/tag/aplysia CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/brembs/article/1023152 <i>Cell, Vol. 127, No. 7. (29 December 2006), pp. 1453-1467.</i><br /><br />SummaryMolecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex--by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals. Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry Leonid Moroz John Edwards Sathyanarayanan Puthanveettil Andrea Kohn Thomas Ha Andreas Heyland Bjarne Knudsen Anuj Sahni Fahong Yu Li Liu doi:10.1016/j.cell.2006.09.052 Cell, Vol. 127, No. 7. (29 December 2006), pp. 1453-1467. 2007-01-03T10:18:42-00:00 2006 Cell 127 7 1453 1467 aplysia classical conditioning instrumental invertebrate neurobiology neuroscience operant pavlovian http://www.citeulike.org/user/brembs/article/877189 <i>Science, Vol. 296, No. 5573. (31 May 2002), pp. 1706-1709.</i><br /><br />Operant conditioning is a form of associative learning through which an animal learns about the consequences of its behavior. Here, we report an appetitive operant conditioning procedure in Aplysia that induces long-term memory. Biophysical changes that accompanied the memory were found in an identified neuron (cell B51) that is considered critical for the expression of behavior that was rewarded. Similar cellular changes in B51 were produced by contingent reinforcement of B51 with dopamine in a single-cell analog of the operant procedure. These findings allow for the detailed analysis of the cellular and molecular processes underlying operant conditioning. Operant reward learning in Aplysia: neuronal correlates and mechanisms. B Brembs FD Lorenzetti FD Reyes DA Baxter JH Byrne doi:10.1126/science.1069434 Science, Vol. 296, No. 5573. (31 May 2002), pp. 1706-1709. 2006-09-29T06:48:42-00:00 2002 Science 1095-9203 296 5573 1706 1709 aplysia conditioning learning memory neurobiology neuroscience operant physiology slug snail http://www.citeulike.org/user/brembs/article/877188 <i>Curr Opin Neurobiol, Vol. 13, No. 6. (December 2003), pp. 710-717.</i><br /><br />Learning to anticipate future events on the basis of past experience with the consequences of one's own behavior (operant conditioning) is a simple form of learning that humans share with most other animals, including invertebrates. Three model organisms have recently made significant contributions towards a mechanistic model of operant conditioning, because of their special technical advantages. Research using the fruit fly Drosophila melanogaster implicated the ignorant gene in operant conditioning in the heat-box, research on the sea slug Aplysia californica contributed a cellular mechanism of behavior selection at a convergence point of operant behavior and reward, and research on the pond snail Lymnaea stagnalis elucidated the role of a behavior-initiating neuron in operant conditioning. These insights demonstrate the usefulness of a variety of invertebrate model systems to complement and stimulate research in vertebrates. Operant conditioning in invertebrates. B Brembs Curr Opin Neurobiol, Vol. 13, No. 6. (December 2003), pp. 710-717. 2006-09-29T06:48:41-00:00 2003 Curr Opin Neurobiol 0959-4388 13 6 710 717 aplysia drosophila flies learning memory neurobiology neuroscience snails http://www.citeulike.org/user/brembs/article/877187 <i>Learn Mem, Vol. 11, No. 4. (g 2004), pp. 412-420.</i><br /><br />Operant and classical conditioning are major processes shaping behavioral responses in all animals. Although the understanding of the mechanisms of classical conditioning has expanded significantly, the understanding of the mechanisms of operant conditioning is more limited. Recent developments in Aplysia are helping to narrow the gap in the level of understanding between operant and classical conditioning, and have raised the possibility of studying the neuronal processes underlying the interaction of operant and classical components in a relatively complex learning task. In the present study, we describe a first step toward realizing this goal, by developing a single in vitro preparation in which both operant and classical conditioning can be studied concurrently. The new paradigm reproduced previously published results, even under more conservative and homogenous selection criteria and tonic stimulation regime. Moreover, the observed learning was resistant to delay, shortening, and signaling of reinforcement. Extending in vitro conditioning in Aplysia to analyze operant and classical processes in the same preparation. B Brembs DA Baxter JH Byrne doi:10.1101/lm.74404 Learn Mem, Vol. 11, No. 4. (g 2004), pp. 412-420. 2006-09-29T06:48:39-00:00 2004 Learn Mem 1072-0502 11 4 412 420 aplysia conditioning learning memory neurobiology neuroscience operant physiology slug snail