CiteULike: 著者: Betker

De Novo Computational Design of Retro-Aldol Enzymes

Lin Jiang — 2008-03-07T02:58:37-00:00

Science, Vol. 319, No. 5868. (7 March 2008), pp. 1387-1391.

The creation of enzymes capable of catalyzing any desired chemical reaction is a grand challenge for computational protein design. Using new algorithms that rely on hashing techniques to construct active sites for multistep reactions, we designed retro-aldolases that use four different catalytic motifs to catalyze the breaking of a carbon-carbon bond in a nonnatural substrate. Of the 72 designs that were experimentally characterized, 32, spanning a range of protein folds, had detectable retro-aldolase activity. Designs that used an explicit water molecule to mediate proton shuffling were significantly more successful, with rate accelerations of up to four orders of magnitude and multiple turnovers, than those involving charged side-chain networks. The atomic accuracy of the design process was confirmed by the x-ray crystal structure of active designs embedded in two protein scaffolds, both of which were nearly superimposable on the design model. 10.1126/science.1152692

Kemp elimination catalysts by computational enzyme design

Daniela Röthlisberger — 2008-03-21T04:33:18-00:00

Nature (19 March 2008)

Okologische Stadterneuerung: Ein neues Leitbild der Stadtentwicklung? : mit einer Fallstudie zur kommunalen Planung in Saarbrucken (Werkberichte / Lehrstuhl fur Planungstheorie Aachen)

Frank Betker — 2008-03-13T13:52:07-00:00

Game-based Exercises for Dynamic Short-Sitting Balance Rehabilitation of People With Chronic Spinal Cord and Traumatic Brain Injuries

Aimee Betker — 2007-11-21T14:07:04-00:00

PHYS THER, Vol. 87, No. 10. (1 October 2007), pp. 1389-1398.

Background and Purpose: Goal-oriented, task-specific training has been shown to improve function; however, it can be difficult to maintain patient interest. This report describes a rehabilitation protocol for the maintenance of balance in a short-sitting position following spinal cord and head injuries by use of a center-of-pressurecontrolled video gamebased tool. The scientific justification for the selected treatment is discussed. Case Descriptions: Three adults were treated: 1 young adult with spina bifida (T10 and L1L2), 1 middle-aged adult with complete paraplegia (complete lesion at T11L1), and 1 middle-aged adult with traumatic brain injury. All patients used wheelchairs full-time. Outcomes: The patients showed increased motivation to perform the game-based exercises and increased dynamic short-sitting balance. Discussion: The patients exhibited increases in practice volume and attention span during training with the game-based tool. In addition, they demonstrated substantial improvements in dynamic balance control. These observations indicate that a video gamebased exercise approach can have a substantial positive effect by improving dynamic short-sitting balance. 10.2522/ptj.20060229