Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease

@article{citeulike:2622985, abstract = {Recombinant HIV-Tat (Tat) induces extensive apoptosis in peripheral blood mononuclear cells (PBMCs) cultured in typical CO2 incubators, which are equilibrated with air (21\% O2). However, as we show here, Tat apoptosis induction fails in PBMCs cultured at physiological oxygen levels (5\% O2). Under these conditions, Tat induces PBMCs to divide, efficiently primes them for HIV infection, and supports virus production by the infected cells. Furthermore, Tat takes only 2 h to prime PBMCs under these conditions. In contrast, PHA/IL-2, which is widely used to prime cells for HIV infection, takes 23 days. These findings strongly recommend culturing primary cells at physiological oxygen levels. In addition, they suggest HIV-Tat as a key regulator of HIV disease progression. 10.1073/pnas.0712363105}, author = {Sahaf, Bita and Atkuri, Kondala and Heydari, Kartoosh and Malipatlolla, Meena and Rappaport, Jay and Regulier, Emmanuel and Herzenberg, Leonard A. and Herzenberg, Leonore A. }, citeulike-article-id = {2622985}, doi = {10.1073/pnas.0712363105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {hiv, replication}, month = {April}, number = {13}, pages = {5111--5116}, posted-at = {2008-04-02 12:16:08}, priority = {2}, title = {Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease}, url = {http://dx.doi.org/10.1073/pnas.0712363105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:2622985 L3 - citeulike-article-id:2622985 TI - Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease JF - Proceedings of the National Academy of Sciences VL - 105 IS - 13 SP - 5111 EP - 5116 N2 - Recombinant HIV-Tat (Tat) induces extensive apoptosis in peripheral blood mononuclear cells (PBMCs) cultured in typical CO2 incubators, which are equilibrated with air (21% O2). However, as we show here, Tat apoptosis induction fails in PBMCs cultured at physiological oxygen levels (5% O2). Under these conditions, Tat induces PBMCs to divide, efficiently primes them for HIV infection, and supports virus production by the infected cells. Furthermore, Tat takes only 2 h to prime PBMCs under these conditions. In contrast, PHA/IL-2, which is widely used to prime cells for HIV infection, takes 23 days. These findings strongly recommend culturing primary cells at physiological oxygen levels. In addition, they suggest HIV-Tat as a key regulator of HIV disease progression. 10.1073/pnas.0712363105 KW - hiv KW - replication AU - Sahaf, Bita AU - Atkuri, Kondala AU - Heydari, Kartoosh AU - Malipatlolla, Meena AU - Rappaport, Jay AU - Regulier, Emmanuel AU - Herzenberg, Leonard AU - Herzenberg, Leonore PY - 2008/04/01/ UR - http://dx.doi.org/10.1073/pnas.0712363105 ER -