Independent effects of cis- and trans-regulatory variation on gene expression in Drosophila melanogaster

@article{citeulike:2328644, abstract = {Biochemical interactions between cis-regulatory DNA sequences and trans-regulatory gene products suggest that cis- and trans-acting polymorphisms may interact genetically. Here we present a strategy to test this hypothesis by comparing the relative cis-regulatory activity of two alleles in different genetic backgrounds. Of the eight genes surveyed in this study, five were affected by trans-acting variation that altered total transcript levels, two of which were also affected by differences in cis-regulation. The presence of trans-acting variation had no effect on relative cis-regulatory activity, showing that cis-regulatory polymorphisms can function independently of trans-regulatory variation. The frequency of such independent interactions on a genomic scale is yet to be determined. 10.1534/genetics.107.082032}, author = {Wittkopp, Patricia J. and Haerum, Belinda K. and Clark, Andrew G. }, citeulike-article-id = {2328644}, doi = {http://dx.doi.org/10.1534/genetics.107.082032}, journal = {Genetics}, keywords = {cis\_vs\_trans, epistasis, regulatory\_polymorphism}, month = {February}, pages = {genetics.107.082032+}, posted-at = {2008-07-07 22:41:18}, priority = {0}, title = {Independent effects of cis- and trans-regulatory variation on gene expression in Drosophila melanogaster}, url = {http://dx.doi.org/10.1534/genetics.107.082032}, year = {2008} }

TY - JOUR ID - citeulike:2328644 L3 - citeulike-article-id:2328644 TI - Independent effects of cis- and trans-regulatory variation on gene expression in Drosophila melanogaster JF - Genetics SP - genetics.107.082032 N2 - Biochemical interactions between cis-regulatory DNA sequences and trans-regulatory gene products suggest that cis- and trans-acting polymorphisms may interact genetically. Here we present a strategy to test this hypothesis by comparing the relative cis-regulatory activity of two alleles in different genetic backgrounds. Of the eight genes surveyed in this study, five were affected by trans-acting variation that altered total transcript levels, two of which were also affected by differences in cis-regulation. The presence of trans-acting variation had no effect on relative cis-regulatory activity, showing that cis-regulatory polymorphisms can function independently of trans-regulatory variation. The frequency of such independent interactions on a genomic scale is yet to be determined. 10.1534/genetics.107.082032 KW - cis_vs_trans KW - epistasis KW - regulatory_polymorphism AU - Wittkopp, Patricia AU - Haerum, Belinda AU - Clark, Andrew PY - 2008/02/03/ UR - http://dx.doi.org/10.1534/genetics.107.082032 ER -