3D reconstruction of high-resolution STED microscope images.

@article{citeulike:2910458, abstract = {Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focused light generally required the use of two lenses in a 4Pi configuration or exceptionally bright photochromic fluorophores. Here, we describe a simple technical solution for 3D nanoscopy of fixed samples: biological specimens are fluorescently labeled, embedded in a polymer resin, cut into thin sections, and then imaged via STED microscopy with nanoscale resolution. This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes. Microsc. Res. Tech., 2008. (c) 2008 Wiley-Liss, Inc.}, address = {Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\"{o}ttingen, Germany.}, author = {Punge, Annedore and Rizzoli, Silvio O O. and Jahn, Reinhard and Wildanger, Jan Dominik D. and Meyer, Lars and Sch\"{o}nle, Andreas and Kastrup, Lars and Hell, Stefan W W. }, citeulike-article-id = {2910458}, doi = {10.1002/jemt.20602}, issn = {1059-910X}, journal = {Microscopy research and technique}, keywords = {super-resolution}, month = {May}, posted-at = {2008-06-20 18:43:14}, priority = {2}, title = {3D reconstruction of high-resolution STED microscope images.}, url = {http://dx.doi.org/10.1002/jemt.20602}, year = {2008} }

TY - JOUR ID - citeulike:2910458 L3 - citeulike-article-id:2910458 TI - 3D reconstruction of high-resolution STED microscope images. JF - Microscopy research and technique AD - Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany. SN - 1059-910X N2 - Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focused light generally required the use of two lenses in a 4Pi configuration or exceptionally bright photochromic fluorophores. Here, we describe a simple technical solution for 3D nanoscopy of fixed samples: biological specimens are fluorescently labeled, embedded in a polymer resin, cut into thin sections, and then imaged via STED microscopy with nanoscale resolution. This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes. Microsc. Res. Tech., 2008. (c) 2008 Wiley-Liss, Inc. KW - super-resolution AU - Punge, Annedore AU - Rizzoli, Silvio O AU - Jahn, Reinhard AU - Wildanger, Jan Dominik AU - Meyer, Lars AU - Schönle, Andreas AU - Kastrup, Lars AU - Hell, Stefan W PY - 2008/05/30/ UR - http://dx.doi.org/10.1002/jemt.20602 ER -