Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa

@article{citeulike:2902949, abstract = {Abstract: Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species.}, address = {Department of Chemistry and Institute for Nanoscale Physics and Chemistry (INPAC), Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium, and Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan}, author = {Fron, E. and Flors, C. and Schweitzer, G. and Habuchi, S. and Mizuno, H. and Ando, R. and Deschryver, F. C. and Miyawaki, A. and Hofkens, J. }, citeulike-article-id = {2902949}, doi = {10.1021/ja069365v}, journal = {J. Am. Chem. Soc.}, keywords = {gfp, photophysics, photoswitchable}, month = {April}, number = {16}, pages = {4870--4871}, posted-at = {2008-06-17 19:19:29}, priority = {2}, title = {Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa}, url = {http://dx.doi.org/10.1021/ja069365v}, volume = {129}, year = {2007} }

TY - JOUR ID - citeulike:2902949 L3 - citeulike-article-id:2902949 TI - Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa JF - J. Am. Chem. Soc. VL - 129 IS - 16 SP - 4870 EP - 4871 AD - Department of Chemistry and Institute for Nanoscale Physics and Chemistry (INPAC), Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium, and Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan N2 - Abstract: Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species. KW - gfp KW - photophysics KW - photoswitchable AU - Fron, E AU - Flors, C AU - Schweitzer, G AU - Habuchi, S AU - Mizuno, H AU - Ando, R AU - Deschryver, FC AU - Miyawaki, A AU - Hofkens, J PY - 2007/04/25/ UR - http://dx.doi.org/10.1021/ja069365v ER -