Non-enzymatic Covalent Protein Labeling Using a Reactive Tag

@article{citeulike:2068299, abstract = {Abstract: We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells.}, address = {Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan, and PRESTO (Life Phenomena and Measurement Analysis, JST), Sanbancho, Chiyodaku, Tokyo, 332-0012, Japan}, author = {Nonaka, H. and Tsukiji, S. and Ojida, A. and Hamachi, I. }, citeulike-article-id = {2068299}, doi = {http://dx.doi.org/10.1021/ja074176d}, journal = {J. Am. Chem. Soc.}, keywords = {bioconjugation, proteins}, month = {December}, posted-at = {2007-12-06 18:25:41}, priority = {5}, title = {Non-enzymatic Covalent Protein Labeling Using a Reactive Tag}, url = {http://dx.doi.org/10.1021/ja074176d}, year = {2007} }

TY - JOUR ID - citeulike:2068299 L3 - citeulike-article-id:2068299 TI - Non-enzymatic Covalent Protein Labeling Using a Reactive Tag JF - J. Am. Chem. Soc. AD - Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan, and PRESTO (Life Phenomena and Measurement Analysis, JST), Sanbancho, Chiyodaku, Tokyo, 332-0012, Japan N2 - Abstract: We describe herein a new method for covalent labeling of proteins using a complementary recognition pair of peptide tag and synthetic molecular probe. The rapid and specific covalent labeling of a tag-fused protein was achieved by the reaction on the tag site with the probe through their selective molecular recognition. The advantages of this method involve the facile functional modification and the high labeling specificity of the tag-fused protein, which are demonstrated in the labeling experiments in various conditions even inside cells. KW - bioconjugation KW - proteins AU - Nonaka, H AU - Tsukiji, S AU - Ojida, A AU - Hamachi, I PY - 2007/12/05/ UR - http://dx.doi.org/10.1021/ja074176d ER -