The colorectal microRNAome.

@article{citeulike:525714, abstract = {MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues.}, address = {The Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins University Medical Institutions, Baltimore, MD 21231; Rosetta Genomics, 10 Plaut Street, Science Park, Rehovot 76706, Israel; Ambion Diagnostics, 2130 Woodward Street, Austin, TX 78744; Rosetta Inpharmatics, 401 Terry Avenue North, Seattle, WA 98109.}, author = {Cummins, Jordan M M. and He, Yiping and Leary, Rebecca J J. and Pagliarini, Ray and Diaz, Luis A A. and Sjoblom, Tobias and Barad, Omer and Bentwich, Zvi and Szafranska, Anna E E. and Labourier, Emmanuel and Raymond, Christopher K K. and Roberts, Brian S S. and Juhl, Hartmut and Kinzler, Kenneth W W. and Vogelstein, Bert and Velculescu, Victor E E. }, citeulike-article-id = {525714}, doi = {10.1073/pnas.0511155103}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {experimental, genome, microrna}, month = {February}, posted-at = {2006-03-02 01:05:50}, priority = {2}, title = {The colorectal microRNAome.}, url = {http://dx.doi.org/10.1073/pnas.0511155103}, year = {2006} }

TY - JOUR ID - citeulike:525714 L3 - citeulike-article-id:525714 TI - The colorectal microRNAome. JF - Proc Natl Acad Sci U S A AD - The Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins University Medical Institutions, Baltimore, MD 21231; Rosetta Genomics, 10 Plaut Street, Science Park, Rehovot 76706, Israel; Ambion Diagnostics, 2130 Woodward Street, Austin, TX 78744; Rosetta Inpharmatics, 401 Terry Avenue North, Seattle, WA 98109. SN - 0027-8424 N2 - MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues. KW - experimental KW - genome KW - microrna AU - Cummins, Jordan M AU - He, Yiping AU - Leary, Rebecca J AU - Pagliarini, Ray AU - Diaz, Luis A AU - Sjoblom, Tobias AU - Barad, Omer AU - Bentwich, Zvi AU - Szafranska, Anna E AU - Labourier, Emmanuel AU - Raymond, Christopher K AU - Roberts, Brian S AU - Juhl, Hartmut AU - Kinzler, Kenneth W AU - Vogelstein, Bert AU - Velculescu, Victor E PY - 2006/02/27/ UR - http://dx.doi.org/10.1073/pnas.0511155103 ER -